A11971 [HAMAMATSU]
Optical Components for Optical Measurement;
| 型号： | A11971 |
| 厂家： | 
HAMAMATSU CORPORATION |
| 描述： | Optical Components for Optical Measurement |
| 文件： | 总28页 (文件大小：1857K) |
| 中文： | 中文翻译 | 下载： | 下载PDF数据表文档文件 |
OPTO
MECHANICS OPTICAL BLOCKS
Optical Components for Optical Measurement
OVERVIEW
FEATURES
The optical blocks are compact units that contain components such
as bandpass filters and dichroic mirrors. They are designed for low-
light-level measurements that use PMT modules and high-sensitivity
cameras. Because their optical components are placed precisely
through the use of high-precision machining, the blocks shield light
superbly. Also, you can use the separate blocks in different combina-
tions by removing and attaching them with thumbscrews.
GEasy to Attach / Detach
GNo Alignment Required
G
Superb Light-shielding Effect
GMakes Low-Light-Level
Measurement Easy
COMPARISON WITH BASIC OPTICAL DEVICES
Mechanics
Light shield
Darkroom
environment
Holder
Integration
Stage
Optics
Filter
Detector
PMT
Mirror
Lens
Photodiode
Camera
LINE UP
Photo
Product Name / Type No.
Over View
Page
4
The A10030 series is an adapter block for connecting a PMT module to a V-
groove type optical block.
Adapter Block
A10030 Series
C-Mount
Adapter Block
A10039
This block connects a device having C-mount to V-groove type optical
blocks. The connection angle for the device and block is adjustable.
4
5
Optical fiber cables with FC or SMA connectors can be connected to the
blocks in the A10037 series.
The lens assembled in the block collimates the light spread from the optical fiber.
Fiber Adapter Block
A10037 Series
This optical block widens the diameter of a collimated beam of light to 2.5
times its original size or narrows the diameter to 1/2.5 times its original size.
Beam Expander Block
A10031
5
The A10038 series is a joint block for connecting one optical block to
another. The joint is available in two types: MM (male-male) type and FF
(female-female) type.
Joint Block
A10038 Series
6
This holder block is a small dark box specifically designed for cuvettes with
an optical path length of 10 mm, and allows easy measurement of samples
in combination with various optical blocks.
Cuvette Holder Block
A11971
6
The A10036 is a manual shutter block with a plate that is opened and
closed by sliding it left and right. Light is sufficiently blocked so there will be
no problem even at photon counting levels.
Shutter Block
A10036
7
A neutral density (ND) filter is included in this optical block to attenuate light
levels.
ND Filter Block
A10032 Series
7
This optical block contains a filter that passes only particular wavelengths.
Bandpass filters and long-pass filters are available.
Filter Block
A10033 Series
8
Interchangeable
Filter Block
A10033-90
The A10033-90 is a holder block for assembling a commercially available
15 mm diameter filter.
9
This block contains a dichroic mirror that reflects particular wavelengths but
passes other wavelengths. A total of six products are available.
Dichroic Block
A10034 Series
10
11
11
12, 13
14
15
16
17
This optical block uses a cubic type half-mirror. Incident light is split into
reflected and transmitted beams at a ratio of 1 to 1.
Beam Splitter Block
A10035
Beam Splitter
Holder Block
A10035-90
The A10035-90 is a holder block designed to hold a 15 mm cubic type
beam splitter.
This is a dark box equipped with 2 micrometers for positionings. It allows
light measurements even under room lighting by externally connecting vari-
ous types of optical blocks.
Stage Block
A10858
This optical block is for using an infinity-corrected objective lens to guide
laser light into an optical fiber with an attached FC connector or to collimate
and transmit the beam from a fiber-optic cable.
Beam Aligner Block
A10760
This block contains a focusing lens with a focal distance of 100 mm and is
used for an infinity-corrected objective lens. Aberration is reduced due to
the use of an achromatic lens.
Tube Lens Block
A10859 Series
This is a feed screw type holder block that can hold a commercially available,
mount type pinhole and adjust its position in the XY direction. Light passing
through the pinhole is collimated by the lens installed in the block and is output.
Pinhole Holder Block
A11027
C-Mount Interchangeable
Filter Block
A11213 Series
The A11213 series is a C-mount connection block for installing a commercial-
ly available 25 mm diameter filter or lens. This block allows angle adjustment
and so is useful as a spacer or adapter that connects between C-mounts.
2
Photo
Product Name / Type No.
Over View
Page
17
This ring is for connecting a PMT module to a optical block and an equip-
ment with C-mount.
C-Mount Ring
A9865
C-Mount Interchangeable
Dichroic Block
A11214
The A11214 is a C-mount connection block for installing a dichroic mirror for
use at a 45° angle of incidence.
18
19
19
This is a holder block designed for a polarizer.
Polarizing filters and waveplates are polarizing elements whose polarization
characteristics change depending on direction and angle.
Polarizer Holder Block
A11026
This maintenance kit includes a set of screws and O-rings needed to as-
semble optical blocks.
Parts Set
A10455-02
CONNECTION EXAMPLES
Page
GConnection Example Using Bifurcated Optical Fiber ......................................................................................................................... 20
GConnection Example Using the Beam Expander Block ..................................................................................................................... 20
GConnecting to a Microscope............................................................................................................................................................... 20
GFluorescence Detection (Confocal) .................................................................................................................................................... 21
GConnection Example Using an Objective Lens and the Tube Lens Block ..................................................................................................... 21
GImaging System Connection Example ............................................................................................................................................... 22
GEmission Detection............................................................................................................................................................................. 23
GExample of Connection to a Mini-Spectrometer................................................................................................................................. 23
G
Simultaneous Measurement with a PMT Module and an Imaging Camera ..................................................................................................... 24
Fluorescence Dye and Connecting Examples ..................................................................................................................................... 25
G
COMPATIBLE PMT MODULE
Screw connection with PMT module
Optical block with
V-groove connector
H10720/H10721
H10722/H10723
H10706/H12056
Adapter Block
A10030-01
H10682
H11890
H11526
H10799
(built-in photodiode)
Optical block with C-mounts
C-Mount Ring
A9865
H7826/H7827/H7828
Optical block with
V-groove connector
Adapter Block
A10030-02
C-mount connection with PMT module
Optical block with
V-groove connector
H7421/H7422
C-Mount Adapter Block
A10039
Optical block with C-mounts
C-mount adapter plate*
H11461/H11462/H8259
* In preparation for use with optical blocks, a C-mount adapter plate must be attached to the PMT module.
Please notify us of your need for this adapter when ordering.
3
BLOCK CHARACTERISTICS
GAdapter Block A10030-01, -02
The A10030-01 is an adapter block for connecting a PMT module to
each block. The A10030-01 adapter block has M4 and M3 screw holes
for securing to an optical bench or optical stage. A mini-post can also
be attached to the M4 screw holes.
Applicable mini-post: OptoSigma Model 155-0XXX
SPECIFICATIONS
Type No.
A10030-01
A10030-02
Weight
Effective Beam Size
8 mm
Approx. 18 g
12 mm*
* When connected to other optical blocks, their effective optical path diameters
take precedence.
Dimensional Outlines (Unit: mm)
A10030-01
26.0 0.1
GASKET
10.8 0.1
15.0 0.1
NOTE: Avoid frequently attaching and detaching from PMT
module.
4-M2
(THUMB SCREW
FOR PMT MODULE)
COMPATIBLE PMT MODULES
H10720/H10721/H10722/H10723/H11706
A10030-01 H12056/H10682/H11890/H11526
H10799 (built-in photodiode)
2-M3 DEPTH 3
(MOUNTING THREADED HOLE)
M4 DEPTH 5
(MOUNTING THREADED HOLE)
0.5
A10030-02 H7826/H7827/H7828
8
4
TPMOA0050EA
A10030-02
27.0 0.1
18.0 0.1
15.5 0.1
GASKET
10.5 0.1
4-M2
(THUMB SCREW
FOR PMT MODULE)
2-M3 DEPTH 4
(MOUNTING THREADED HOLE)
M4 DEPTH 5
(MOUNTING THREADED HOLE)
0.5
8
4
TPMOA0085EA
GC-Mount Adapter Block A10039
One end of this adapter block has a C-mount (male threads) that con-
nects to the H7421 series or H7422 series PMT module, while the oth-
er end couples to another optical block. This adapter block also con-
nects to a commercially available device with a C-mount.
This adapter block can also be connected to commercially sold C-
mount products.
SPECIFICATIONS
Type No.
Weight
Effective Beam Size
8 mm
A10039
Approx. 27 g
* Supplied: C-mount double female thread ring.
Dimensional Outlines (Unit: mm)
C-MOUNT (MALE)
4
How to connect a C-mount adapter block
22.0 0.1
16.5 0.2
3.5 0.1
TACCA0287EA
3. Turn the adapter block
body counterclockwise
so that it is positioned
at the desired angle
and then tighten the
four screws.
1. Loosen the four screws 2. Insert the C-mount ring
on the adapter block
and make sure the C-
mount ring is separated
about 1 mm from the
adapter block body.
into the C-mount of the
PMT module and turn
the adapter block body
clockwise until it stops.
4
GFiber Adapter Block A10037 Series
Optical fiber cables with FC or SMA connectors can be connected to
the blocks in the A10037 series.
The lens assembled in the block collimates the light spread from the
optical fiber.
The A10037/A10037-01 is suitable for use in the visible region (400 nm
to 700 nm) and the A10037-10/A10037-11 for use in the UV region
(200 nm to 400 nm).
SPECIFICATIONS
Type No.
Recommended Wavelength Connecter Focal Length
Weight
A10037
FC
Visible
SMA
10 mm
FC
A10037-01
A10037-10
A10037-11
Approx. 17 g
UV
LA10037
LA10037-01
SMA
Dimensional Outlines (Unit: mm)
Transmittance (Typical)
22.0 0.1
7.0 0.1
(8)
TACCB0094EB
100
80
60
40
20
0
A10037/-10
A10037-01/-11: L=13.5
: L=9.0
L
When fiber core diameter is 100 µm and NA is 0.2
Distance from Fiber Output End
Beam Diameter
Max. 4.9 mm
Max. 5.4 mm
100 mm
150 mm
A10037 / A10037-01
A10037-10 / A10037-11
* In the case of the SMA connector type, the beam diameter
depends on the position of the connected fiber cable end.
The above values are measured when the fiber cable end is
at the same position as the FC connector type.
200
300
400
500
600
700
800
WAVELENGTH (nm)
TACCA0270EB
GBeam Expander Block A10031
This optical block widens the diameter of a collimated beam of light to 2.5
times its original size or narrows the diameter to 1/2.5 times its original
size. This block has a C-mount thread for connecting to another device.
SPECIFICATIONS
Type No.
Weight
Effective Beam Size
8 mm
A10031
Approx. 43 g
* Supplied: C-mount double male thread ring.
** Designed for wavelength from 400 nm to 700 nm.
Dimensional Outlines (Unit: mm)
Transmittance (Typical)
TACCB0095EB
100
80
60
40
20
0
C-MOUNT (FEMALE)
44.4 0.3
8
8
TACCA0271EA
Incident light conditions
44.4 0.3
300
400
500
600
700
800
WAVELENGTH (nm)
TACCC0127EA
5
BLOCK CHARACTERISTICS
GJoint Block A10038 Series
The A10038 series is a joint block for connecting one optical block to
another. The joint is available in two types: MM (male-male) type and FF
(female-female) type. Interposing the MM block (A10038-01) and/or FF
block (A10038-02) between optical blocks allows various connections.
SPECIFICATIONS
Type No.
A10038-01
A10038-02
Effective Beam Size
Weight
8 mm
8 mm
Approx. 10 g
Approx. 25 g
Dimensional Outlines (Unit: mm)
A10038-01
5.0 0.1
LA10038-01
(8)
(8)
22.0 0.1
TACCA0284EA
A10038-02
22.0 0.1
19.0 0.2
TACCA0285EA
LA10038-02
GCuvette Holder Block A11971
The A11971 cuvette holder block is a small dark box specifically
designed for cuvettes with an optical path length of 10 mm. It
allows easy measurement of samples in combination with other
optical blocks. The base can be removed as needed.
NOTE: This cuvette holder block is very compact. If it falls over, the sample
may leak from the cuvette, so we recommend securing the body of
the holder block and / or using a capped cuvette.
SPECIFICATIONS
Applicable Cuvette Size
Effective
Beam Size
Type No.
Weight
Optical Path Length Dimensional Outline
CUVETTE HOLDER BLOCK
12.5 mm ×
56 mm (H) (including the base)
Approx. 206 g
A11971
8 mm
10 mm
CUVETTE HOLDER BLOCK + CUVETTE
NOTE: Cuvette is sold separately.
Connection Example: Absorbance and Side
Fluorescence Detection Using Optical Fiber
Dimensional Outlines (Unit: mm)
(25)
28.1
(10)
DASHED LINE CUVETTE
19.5
38.2 1.0
21
ADAPTER BLOCK A10030-01
PMT MODULE
BLOCK CAP
25.0 0.1
8.1
18.0 0.1
PHOTOSENSOR MODULE
H10799-xxx
2
BLOCK CAP
22
A
A'
2 × 4.5
BASE
(REMOVABLE)
FILTER BLOCK
A10033-xx
(FOR HOLDING
THE BASE IN
PLACE)
32
CROSS SECTION A–A'
(WHEN BLOCK CAP IS REMOVED)
15.0 0.1
30
BASE MOUNTING SURFACE
M3
FIBER ADAPTER
50.0 0.1
60
BLOCK
A10037-xx
BOLTS WITH HEXAGONAL HOLES
FOR ATTACHING THE BASE
CUVETTE HOLDER
BLOCK
TPMOA0086EA
A11971
6
GShutter Block A10036
The A10036 is a manual shutter block with a plate that is opened and
closed by sliding it left and right. Light is sufficiently blocked so there
will be no problem even at photon counting levels.
SPECIFICATIONS
Type No.
Weight
Effective Beam Size
8 mm
A10036
Approx. 48 g
Dimensional Outlines (Unit: mm)
NOTE: If you feel the shutter does not open and close
smoothly, we recommend applying small amount of
an optical grease to the plate.
2
16.0 0.1
30.0 0.1
(8)
TACCA0288EA
GND Filter Block A10032 Series
A neutral density (ND) filter is included in this optical block to attenuate
light levels.
SPECIFICATIONS
Effective Beam Size
8 mm
Type No.
A10032-10
A10032-11
Weight
Built-in Filter Transmittance
10 %
ND Filter
Approx. 26 g
1 %
*
Light transmittance is the average transmittance at wavelengths from 400 nm to 700 nm.
Dimensional Outlines (Unit: mm)
20.0 0.1 (8)
2
Transmittance (Typical)
TACCB0087EA
80
22.0 0.1
60
40
A10032-10
A10032-11
20
0
TACCA0272EA
300
400
500
WAVELENGTH (nm)
600
700
7
BLOCK CHARACTERISTICS
GFilter Block A10033 Series
This optical block contains a filter that passes only particular
wavelengths. Bandpass filters and long-pass filters are available.
If you would like to use a filter with specifications other than those
described below, purchase the A10033-90 interchangeable filter block
(p. 9), which does not come with a preset filter. The A11213 C-mount
interchangeable filter block series (p. 17) is also available. Filters with
25 mm diameters can be installed in these blocks.
SPECIFICATIONS
Type No.
A10033-01
A10033-02
A10033-03
A10033-04
A10033-05
A10033-06
Built-in Filter
Central Wavelength
530 nm 4 nm
610 nm 4 nm
630 nm 5 nm
460 nm 4 nm
575 nm 4 nm
700 nm 5 nm
FWHM
Effective Beam Size
8 mm
Weight
40 nm 8 nm
40 nm 8 nm
60 nm 10 nm
60 nm 8 nm
30 nm 8 nm
60 nm 10 nm
Bandpass Filter
Approx. 25 g
* Stopband: Below 0.01 % at 300 nm to 950 nm (except transmission bands)
Type No.
A10033-61
A10033-62
A10033-63
A10033-71
Built-in Filter
Cut-on Wavelength Effective Beam Size
390 nm
Weight
500 nm
8 mm
Longpass Filter
Approx. 25 g
600 nm
510 nm
Transmittance [Bandpass filter] (Typical)
A10033-01
A10033-02
A10033-03
TACCB0077EA
100
TACCB0078EA
TACCB0079EA
100
100
90
80
70
60
50
40
30
20
10
0
90
80
70
60
50
40
30
20
10
0
90
80
70
60
50
40
30
20
10
0
300
400
500
600
700
800
300
400
500
600
700
800
300
400
500
600
700
800
WAVELENGTH (nm)
WAVELENGTH (nm)
WAVELENGTH (nm)
A10033-04
A10033-05
A10033-06
TACCB0080EA
100
TACCB0081EA
100
TACCB0082EA
100
90
80
70
60
50
40
30
20
10
0
90
80
70
60
50
40
30
20
10
0
90
80
70
60
50
40
30
20
10
0
300
400
500
600
700
800
300
400
500
600
700
800
300
400
500
600
700
800
WAVELENGTH (nm)
WAVELENGTH (nm)
WAVELENGTH (nm)
8
Transmittance [Longpass filter] (Typical)
A10033-61, A10033-62, A10033-63 (Colored glass filter)
A10033-71
TPMOB0214EA
TACCB0083EA
100
100
90
80
70
60
50
40
30
20
10
0
90
80
A10033-61
A10033-62
A10033-63
70
60
50
40
30
20
10
0
300
400
500
600
700
800
300
400
500
600
700
800
WAVELENGTH (nm)
WAVELENGTH (nm)
* Transmittance characteristics are typical examples and
may slightly vary depending on production lots.
* Dimensional outlines are the same as the A10033-90.
G
Interchangeable Filter Block A10033-90
How to install an optical filter
The A10033-90 is a filter holder block that allows you to insert an
optical filter according to your application. The filter is held by the
spacer ring and the retaining ring, and can be easily exchanged by
using the accessory tool.
SPECIFICATIONS
Effective
Beam Size
1. Loosen the thumbscrews.
2. Insert, in order, the optical filter,
spacer ring and retaining ring.
Type No.
Conpatible Filter Size
Weight
Diameter: 12.7 mm (1/2 inch) to 15 mm (Max.)
(recommended: 15 mm)
Thickness: 1.5 mm to 5 mm
Approx.
24 g
A10033-90
8 mm
Dimensional Outlines (Unit: mm)
20.0 0.1 (8)
2
3. Tighten the retaining ring with
the tightening tool.
4. Install the O-ring in place.
•
NOTE: Filters for this block must be purchased separately.
•
Do not install optical filters in the wrong direction be-
cause they have the front side and back side for an
light beam. Always insert the O-ring for blocking light
after installing an optical filter.
•
When installing an optical filter, be careful to keep it
22.0 0.1
clean and to avoid getting fingerprints on it.
TACCA0272EA
Connection Example: Fiber-Optic Single Wavelength Detection
The lens inside the A10037 fiber adapter block collimates the beam of the light signal emitted from the optical fiber. The A10033 filter
block only allows a specific wavelength to pass. When dimming the beam, use ND filter block A10032.
ND FILTER BLOCK
A10032-xx
FILTER BLOCK
A10033-xx
FILTER BLOCK
A10033-xx
PMT MODULE
ADAPTER BLOCK A10030-01
PMT MODULE
ADAPTER BLOCK
A10030-01
FIBER ADAPTER BLOCK
A10037-xx
FIBER ADAPTER BLOCK A10037-xx
TPMOC0211EA
9
BLOCK CHARACTERISTICS
GDichroic Block A10034 Series
This optical block contains a dichroic mirror that reflects particular
wavelengths but transmits other wavelengths.
If you would like to use a mirror with specifications other than those
described below, purchase the A11214 C-mount interchangeable
dichroic block (p. 18), which does not come with a preset mirror.
SPECIFICATIONS
Built-in Dichroic
Effective
Beam Size
Type No.
Weight
Mirror
A10034-01
A10034-02
A10034-03
A10034-04
A10034-05
A10034-06
DM505
DM565
DM600
DM660
DM430
DM455
8 mm
Approx. 57 g
NOTE: • Ultraviolet light shorter than 380 nm is absorbed by the
coating materials deposited on the dichroic mirror, so the
reflectance and transmittance significantly decrease.
•
For the reflected light, use the wavelength range where
light is reflected more than 95 % (see the figures below).
Transmittance (Typical)
A10034-01
A10034-02
A10034-03
TACCB0086EA
TACCB0084EA
100
TACCB0085EA
100
100
90
80
70
60
50
40
30
20
10
0
Reflectance 95 % or more
at 540 nm to 575 nm
Reflectance 95 % or more
at 510 nm to 545 nm
Reflectance 95 % or more
90
90
80
70
60
50
40
30
20
10
0
at 445 nm to 480 nm
80
70
60
50
40
30
20
10
0
300
400
500
600
700
800
300
400
500
600
700
800
300
400
500
600
700
800
WAVELENGTH (nm)
WAVELENGTH (nm)
WAVELENGTH (nm)
A10034-04
A10034-05
A10034-06
TACCB0093EA
100
TACCB0096EA
100
TACCB0097EA
100
Reflectance 95 % or more
at 460 nm to 625 nm
90
80
70
60
50
40
30
20
10
0
90
80
70
60
50
40
30
20
10
0
90
80
70
60
50
40
30
20
10
0
Reflectance 95 % or more
at 380 nm to 405 nm
Reflectance 95 % or more
at 385 nm to 420 nm
300
400
500
600
700
800
300
400
500
600
700
800
300
400
500
600
700
800
WAVELENGTH (nm)
WAVELENGTH (nm)
WAVELENGTH (nm)
* Dimensional outlines are the same as the A10035 (see the dimensional outlines on the next page).
Connection Example: Fiber-Opic Dual Wavelength Detection
PMT MODULE
The lens inside the A10037 fiber adapter block collimates the beam
of the light signal emitted from the optical fiber. The dichroic mirror
inside the A10034 dichroic block reflects light below a certain
wavelength and passes light above that wavelength. Finally, each of
the two A10033 filter blocks isolates a specific wavelength so that the
signal levels of the two separate wavelengths can be measured.
FILTER BLOCK
A10033-xx
ADAPTER BLOCK
A10030-01
ADAPTER BLOCK
A10030-01
DICHROIC BLOCK
A10034-xx
FIBER ADAPTER BLOCK
A10037-xx
10
GBeam Splitter Block A10035
This optical block uses a cubic type half-mirror. Incident light is split into
reflected and transmitted beams at a ratio of 1 to 1.
SPECIFICATIONS
Type No.
Effective Beam Size
8 mm
Weight
A10035
Approx. 63 g
* Designed for wavelength from 400 nm to 700 nm.
Dimensional Outlines (Unit: mm)
2
NOTE: Light level is cut to approximately one third due to light
absorption.
Transmittance (Typical)
( 16)
TACCB0089EC
100
9.0 0.1
80
60
P
AVERAGE
40
20
22.0 0.1
37.0 0.2
(8)
TACCA0273EA
S
0
400
450
500
550
600
650
700
WAVELENGTH (nm)
G
Beam Splitter Holder Block A10035-90
The A10035-90 is a holder block designed to hold a 15 mm cubic type
beam splitter.
SPECIFICATIONS
Effective
Beam Size
Type No.
Applicable Beam Splitter Size
Weight
A10035-90
8 mm
15 mm × 15 mm × 15 mm cubic type Approx. 55 g
* Supplied: Hex wrench
Dimensional Outlines (Unit: mm)
2
26.0
16)
DASHED LINE
APPLICABLE BEAM SPLITTER SIZE
15 × 15 × 15
NOTE: • The beam splitter is not included.
• Be careful to install the beam splitter so that it faces
in the correct direction.
(
4-M3 DEPTH 6
(FOR HOLDING THE
BLOCK IN PLACE)
9.0 0.1
• When installing a beam splitter, be careful to keep it
clean and to avoid getting fingerprints on it.
22.0 0.1 17 0.1
(39)
15.0 0.4
11.0 0.1
20.0 0.1
(8)
(26)
BOTTOM VIEW
TPMOA0068EA
11
BLOCK CHARACTERISTICS
GStage Block A10858
The stage block is for using an infinity-corrected objective lens in object
image generation and fluorescence detection. It has two C-mount ports on
the top and bottom of its rear panel. You can use other optical blocks to
connect the stage block to a PMT module or ICCD camera (p. 27). Because
you can use the stage block cover to keep the measured area in darkness,
the stage block is useful even when detecting faint light. Furthermore, if you
remove the cover and illuminate the sample appropriately using an
illuminator, you can generate images by using the stage block with the
A10859-01 tube lens block, CCD cameras, and other devices.
SPECIFICATIONS
Travels
Compatible
Type No.
Weight
Port
X-Axis
Z-Axis
Objective Lens
Infinity-corrected Objective lens
M20.32 × P0.706
Parfocal distance*: 45 mm
Approx.
4 kg
A10858
6.5 mm
3 mm 2; C-mount
* For information about the parfocal distance, see p. 14.
Dimensional Outlines (Unit: mm)
110
50
(38)
160
2-C-MOUNT
Directions that you can
move the workplate in
using a micrometer.
Direction of beam
movement caused
by beam steering
mirror assembly
adjustment
TPMOA0049EA
Workplate and Beam Movement
From outside of the stage block, you can use a micrometer to move the
workplate horizontally along the X axis or vertically along the Z axis.
You can also use the three setup screws in the beam steering mirror
assembly to change the internal mirror angle and adjust the horizontal
Y axis.
Beam steering
mirror assembly
setup screws
Accesories
Dimensional Outlines (Unit: mm)
Stage Block Rack A11058
4-M6
204
250
Sizes of the included spacers
Spacer 1
Spacer 2
Spacer 3
90 mm × 90 mm, Thickness: 10 mm
60 mm × 60 mm, Thickness: 18 mm
60 mm × 60 mm, Thickness: 25 mm
2
1
1
HEIGHT-ADJUSTMENT BOLT
The spacer sizes have been designed according to their uses.
TPMOA0066EA
12
Part Names and Features
M6 Mounting Hole
C-Mount Port
Workplate
M6-thread mounting hole. You
can use a mounting post or an
angle mount for illumination.
There are two C-mount ports at
the top and bottom of the stage
block. You can connect the
stage block to other optical
blocks through blocks such
as the A11213 C-mount in-
terchangeable filter block.
The stage block comes with three different
workplates:
A workplate with a 36 mm diam-
eter hole. With this workplate,
you can put the sample on a
slide and observe its reflected-
light or transmitted-light image
or measure its fluorescence.
This workplate is specially de-
signed for use with the
A10657-01 microwell slide
(sold separately).
A workplate that you can
place an object on to observe
its reflected-light image in an
upright configuration or to ex-
cite it from above and meas-
ure its fluorescence.
* Please purchase illuminators from a
company that manufactures them.
Micrometer
The micrometer enables you
to perform precise horizontal
(X-axis) and vertical (Z-axis)
alignment and to accurately
reproduce prior positionings.
NOTE: Objective lens is not included.
Spacer
Objective Lens Mount A10921
A 20 mm spacer is installed
This is a mount for an objective lens. You can mount and remove
the objective lens mount using the left and right screws. You can
place the objective lens mount at the top of the stage mount for
upright configurations, and at the bottom for inverted configura-
tions. The A10858 comes with one objective lens mount.
between the workplate and
the XZ axis stage. Remov-
ing this spacer will give you
20 mm of extra space,
which is useful when ob-
serving relatively tall objects
in an upright configuration.
You can move the work-
plate along the X and Y ax-
es by attaching an X-axis
stage (SURUGA SEIKI
B11-40C) in place of the
spacer.*
45 mm
Parfocal distance
45 mm
Micrometer height
adjustment of
3 mm
NOTE: An additional A10921 objective lens mount (sold
separately) is attached in the above example.
Stage clips are not included.
* Please direct inquiries about
the X-axis stage to SURUGA
SEIKI.
Accesories
Objective Lens Mount A10921
Microwell Slide A10657-01
* There is a separate catalog for microwell slides.
Please refer to that catalog for more information.
13
BLOCK CHARACTERISTICS
GBeam Aligner Block A10760
This optical block is for using an infinity-corrected objective lens to
guide laser light into an optical fiber with an attached FC connector or
to collimate and transmit the beam from a fiber-optic cable.
The block has two micrometers for positioning the optical fiber along
the X and Y axes and an additional micrometer at the bottom for fine
adjustments along the Z axis.
This block has a cover around the objective lens and an additional
cover for the entire block that further increases its light shielding
capability.
SPECIFICATIONS
Travels
Optical Fiber
Compatible
Type No.
Weight
XY-Axis Z-Axis Connector
Objective Lens
Infinity-corrected Objective lens
M20.32 × P0.706
Parfocal distance: 45 mm
Approx.
420 g
A10760
1 mm
3 mm
FC
NOTE: Objective lens and fiber-optic cable are not included.
* Supplied: Hex wrench
USING EXAMPLE 1: FOCUSING
BEAM ALIGNER BLOCK
A10760
Dimensional Outlines (Unit: mm)
OBJECTIVE LENS
SCREW
OPTICAL FIBER
C-MOUNT (FEMALE)
INFINITY-CORRECTED
OBJECTIVE LENS
TPMOC0209EB
USING EXAMPLE 2: COLLIMATOR
BEAM ALIGNER BLOCK
A10760
OPTICAL FIBER
70
52
INFINITY-CORRECTED
OBJECTIVE LENS
TPMOC0210EA
TPMOA0051EA
Technical Guide
Pupil Diameter and Parfocal Distance
PUPIL DIAMETER
OBJECTIVE
MOUNTING POSITION
1. Remove the two cover screws using a
Phillips-head screwdriver, and then
remove the cover.
2. Use the hexagonal wrench that comes
with the block to remove the two plate
screws, and then remove the plate.
PARFOCAL DISTANCE
Objective lens
Y-axis
adjustment
screw
SAMPLE SURFACE
TPMOC0223EA
Pupil Diameter
X-axis
Z-axis
The diameter of the beam that is emitted from the pupil
of an objective lens with a focal length f and a numerical
aperture NA is given by:
adjustment screw
adjustment screw
3. Attach an objective lens to the plate's
objective lens screw hole (M20.32
P0.706).
4. Reassemble the block by performing
steps 1 and 2 in reverse, and then
connect the block to other devices.
You can align the optical fiber by using
the X-, Y-, and Z-axis adjustment
screws. Only turn the Z-axis adjustment
screw after you have loosened the
screws in step 1.
Pupil diameter = 2 × f × NA
Parfocal Distance
The parfocal distance is the distance from the objective
mounting position of the objective lens to the sample at
which the sample is in-focus. The A10760 is designed to
be used with an objective lens whose parfocal distance
is 45 mm.
14
GTube Lens Block A10859 Series
LA10859
LA10859-01
Transmittance (Typical)
These are video lens blocks for use with the infinity-corrected objective
lenses. It has a tube lens with a focal length of 100 mm to form an
image. One side has a male C-mount thread, and the other side has a
female C-mount thread. You can attach a device such as an ICCD or
CCD camera to the side with the male thread.
You can use a C-mount double male thread ring to connect the side
with the female thread to the A10858 stage block.
The tube lens block reduces aberration through the use of an
achromatic lens.
TPMOB0251EA
100
80
60
40
20
0
SPECIFICATIONS
Recommended
Type No.
A10859
A10859-01
Focal Length Effective Beam Size
Weight
Sensor Size
15 mm
100 mm
1/3 inches or less Approx. 130 g
1/2 inches or less Approx. 205 g
300
400
500
600
700
800
900
1000
24 mm
WAVELENGTH (nm)
* Supplied: C-mount double male thread ring.
* Designed for wavelength from 400 nm to 700 nm.
Dimensional Outlines (Unit: mm)
A10859
A10859-01
MANUAL
FOCUSING RING
CAMERA ANGLE
ADJUSTING SCREW*
CAMERA ANGLE
ADJUSTING SCREW*
MANUAL
FOCUSING RING
C-MOUNT (FEMALE)
C-MOUNT (FEMALE)
C-MOUNT (MALE)
C-MOUNT (MALE)
IMAGE PLANE
IMAGE PLANE
4
4
4
4
93 (92-94)
17.526
96 (95-97)
17.526
TPMOA0052EA
* You can adjust the camera tilt by loosening this screw and turning the tube lens block.
TPMOA0053EA
Technical Guide
Imaging Lens
Distance Between the Imaging Lens and the Objective Lens
The light from the sample is collimated by the infinity-
corrected objective lens before it enters the imaging lens.
The imaging lens focuses the image on the focal point.
Even though the beam of light that passes from the infinity-corrected
objective lens to the imaging lens is collimated, the distance between the
lenses must still be kept below a certain limit. This limit (L) can be
calculated from the specifications of the objective and imaging lenses and
the effective area of the sensor.
COLLIMATED
LIGHT BEAM
CAMERA
where φ1 is the objective lens's exit pupil
diameter (mm), f1 is the focal length (mm), NA
is the numeric aperture, φ2 is the imaging lens
effective diameter, f2 is the focal length (mm),
φ is the effective sensor area, and d is the
observation area.
f2(φ2 – φ1)
L (mm) =
φ
INFINITY-CORRECTED
OBJECTIVE LENS
IMAGING LENS
φ1 = 2 × f1 × NA
TUBE LENS BLOCK
A10859 / A10859-01
TPMOC0224EA
φ
d =
Magnification
M
You can calculate the magnification from the imaging lens
focal length (100 mm) and the focal length of the
microscope's objective lens.
To avoid vignetting when using the A10859 with objective lens (f1 = 18
mm, NA = 0.25) and a 1/3-inch video camera (φ = 6 mm), keep the
distance from the objective lens to the A10859 below 100 mm. At this
distance, placing a dichroic mirror between the objective lens and the
A10859 will not have much of an effect on the overall optical
characteristics.
Imaging lens focal length
Objective lens focal length
100
Magnification (M) =
=
Objective lens focal length
Example: When the objective lens focal length is 45 mm, the
magnification is approximately 2.2 times.
15
BLOCK CHARACTERISTICS
GPinhole Holder Block A11027
The A11027 is a holder block for mounting a pinhole. The screws can
be used to adjust the pinhole's position in the XY directions. The built-in
lens collimates the light that passes through the pinhole and converges
the light on a photodetector.
There is a base attached to the holder block through the M3 screw
holes in the bottom of the block. If necessary, this base can be re-
moved and re-attached to the side of the block.
SPECIFICATIONS
Applicable Pinhole XY Axis Range Adjustment Focal
Type No.
Weight
Mount Size
of Motion
Screw Pitch Length
Approx. 180 g
(including
Diameter: 16 mm
A11027
1.2
0.25 mm 15 mm
Thickness:
1.58 mm to 1.6 mm
NOTE: • The collimate status changes depending on the pin-
hole diameter and the wavelength. (The assumed
wavelength is between 400 nm and 700 nm.)
• The pinhole is not included. Use a pinhole that is in-
stalled in a mount. (Products by companies such as
SIGMA KOKI CO., LTD.; SURUGA SEIKI CO., LTD.;
and CVI Melles Griot can be used.)
the base)
* Supplied: Screw ring tool and hex wrench
Dimensional Outlines (Unit: mm)
(27.526)
8.0
Y-AXIS ADJUSTMENT SCREW
ADJUSTMENT: 1.2
(REFERENCE PLANE OF
THE PINHOLE MOUNT) 17.746
C-MOUNT ANGLE
ADJUSTMENT RING
• Be careful to install the pinhole so that it faces in the
correct direction.
• When installing a pinhole, be careful to keep it clean
and to avoid getting fingerprints on it.
50.0
11.5 1.5
(0.22)
2-M3 DEPTH 3
12.0
(ASSUMED PINHOLE PLANE) 17.526
10.0
(FOR HOLDING THE
BLOCK IN PLACE)
PINHOLE MOUNTING
SCREW RING
X-AXIS ADJUSTMENT
SCREW
ADJUSTMENT: 1.2
Transmittance for the Collimation Lens (Typical)
TPMOB0233EA
100
80
60
40
20
0
C-MOUNT
(FEMALE)
22.95
50.0
APPLICABLE PINHOLE MOUNT SIZE
DIAMETER: 16. THICKNESS: 1.6
BASE (REMOVABLE)
2-M3
BOLTS WITH HEXAGONAL HOLES
FOR ATTACHING THE BASE
30.0
2- 4.5
(FOR HOLDING THE BASE IN PLACE)
M4 DEPTH 5
(FOR HOLDING THE
BLOCK IN PLACE)
60.0
BASE MOUNTING SURFACE
TACCA0070EA
Extended Example of Microscope
Connecting the A11027 pinhole holder block to the C-mount port of a
microscope configures a confocal optical system. Light passing
through the pinhole is collimated by the lens in the block and is effi-
ciently guided to the PMT module.
300
400
500
600
700
800
WAVELENGTH (nm)
FILTER BLOCK A10033-xx
PMT MODULE
ADAPTER BLOCK
A10030-01
PINHOLE HOLDER BLOCK A11027
MICROSCOPE C-MOUNT PORT
MICROSCOPE
16
G
C-Mount Interchangeable Filter Block A11213 Series
These C-mount threaded optical blocks are for installing commercial
optical filters and lenses with 25 mm diameters. You can use it with the
A11214 C-mount interchangeable dichroic block to measure fluores-
cence or perform other tasks.
Because this block enables angle adjustment, it is also useful as a
spacer between C-mounts and as a connection adapter.
SPECIFICATIONS
Effective
Type No.
Compatible Filter Size
Weight
Beam Size
A11213
Diameter: 24 mm to 26 mm (25 mm to 25.4 mm recommended) Approx. 29 g
Thickness: 5 mm (Max.) (2 mm to 5 mm recommended) Approx. 30 g
20 mm
A11213-01
LLeft: A11213, Right: A11213-01
Connection Example: Single Wavelength Detection
Only light at a specific wavelength passes through the optical filter
assembled in the block and is detected by the PMT module.
NOTE: • Filters and lenses for this block must be purchased
separately.
•
When you install a filter that is designed to receive a
beam of light from a specific side, make sure that you
install the filter in the correct direction.
C-MOUNT RING A9865
•
When installing an optical filter or other device, be care-
ful to keep it clean and to avoid getting fingerprints on it.
PMT MODULE
Dimensional Outlines (Unit: mm)
A11213 (Male–Male Type)
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213-xx
C-MOUNT (MALE)
2 (DATUM PLANE OF FILTER)
Connection Example: Fiber-Optic Single Wavelength Detection
Light emitted and spread from the optical fiber cable is collimated by
the lens in the A10037 series fiber adapter block and is efficiently
guided to the PMT module.
4
20
4
C-MOUNT ADAPTER BLOCK
A10039
TPMOA0054EA
A11213-01 (Male–Female Type)
C-MOUNT (FEMALE)
2 (DATUM PLANE OF FILTER)
C-MOUNT (MALE)
PMT MODULE
C-MOUNT RING A9865
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213-01
FIBER ADAPTER BLOCK A10037-xx
5
20
4
TPMOA0055EA
GC-Mount Ring A9865
This ring is for connecting a PMT module to a optical block and an
equipment with C-mount.
SPECIFICATIONS
Type No.
Effective Beam Size
8 mm
Weight
A9865
Approx. 7 g
Dimensional Outlines (Unit: mm)
4- 3.2
4- 2.1
C-MOUNT
GASKET
COMPATIBLE PMT MODULES
H10720/H10721/H10722/H10723/H11706/H12056/H10682
H11890/H11526
5.0
7.0 0.5
14
TPMOA0056EA
H10799 (built-in photodiode)
17
BLOCK CHARACTERISTICS
C-Mount Interchangeable Dichroic Block A11214
G
This C-mount threaded optical block is for installing a dichroic mirror for
use at a 45° angle of incidence. You can use typical dichroic mirrors
that are commercially available for use with microscopes.
SPECIFICATIONS
Effective
Type No.
Compatible Dichroic Mirror Size
Weight
Beam Size
25 mm to 26 mm (W) × 35 mm to 38 mm (H)
Thickness: 0.9 mm to 1.1 mm (1 mm recommended)
Approx.
84 g
A11214
20 mm
* Supplied: Hex wrench
Dimensional Outlines (Unit: mm)
NOTE: • Dichroic mirrors for this block must be purchased sep-
arately.
• Commercially sold dichroic mirrors usually have mark-
ings to distinguish their front and back sides. Be care-
ful to install dichroic mirrors in the A11214 in the cor-
rect direction.
• When installing a dichroic mirror, be careful to keep it
clean and to avoid getting fingerprints on it.
C-MOUNT (FEMALE) DEPTH 5
20
20
C-MOUNT (FEMALE) DEPTH 5
C-MOUNT (FEMALE) DEPTH 5
Connection Example:
Fiber-Optic Dual / Triple Wavelength Detection
The dichroic mirror assembled in the block reflects light
at wavelengths shorter than a specific wavelength and
transmits longer wavelength light. Furthermore, only
light at a specific wavelength passes through the optical
filter and is then detected by the PMT module.
18
18
40
18
18
TPMOA0057EA
Dual Wavelength Detection
PMT MODULE
PMT MODULE
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
C-MOUNT
RING A9865
C-MOUNT
RING A9865
C-MOUNT
INTERCHANGEABLE
DICHROIC BLOCK A11214
FIBER ADAPTER
BLOCK A10037-xx
C-MOUNT ADAPTER BLOCK A10039
Triple Wavelength Detection
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
PMT MODULE
C-MOUNT
RING A9865
C-MOUNT
INTERCHANGEABLE
DICHROIC BLOCK
A11214
C-MOUNT ADAPTER BLOCK A10039
FIBER ADAPTER BLOCK A10037-xx
18
G
Polarizer Holder Block A11026
Polarizing filters and waveplates are polarizing elements whose polari-
zation characteristics change depending on direction and angle. The
A11026 is a simple, course-movement holder block that can be used to
adjust the direction and angle.
SPECIFICATIONS
Effective
Beam Size
Applicable Polarizing
Element Size
Adjustable Angle
Type No.
Weight
Range
Scale
2
Diameter: 25 mm to 25.4 mm
Thickness: 1 mm to 8 mm
5°
1
A11026
8 mm
90°
Approx. 55 g
Intervals
1Can be rotated 90° or more by changing the angle adjustment screw position.
2Scale indicates adjustment intervals and does not indicate adjustable pitch.
Can be fixed at any desired angle within the adjustable range of 90°.
* Supplied: Hex wrench
NOTE: • The polarizing filter or waveplate is not included.
(Products by companies such as Edmund Optics Inc.
and SIGMA KOKI CO., LTD. can be used.)
Dimensional Outlines (Unit: mm)
ANGLE SCALE: 5° INTERVALS
DASHED LINE
APPLICABLE OPTICAL ELEMENT SIZE
DIAMETER: 25.4. THICKNESS: 1 to 8
ANGLE
ADJUSTMENT
SCREW
• When installing a polarizing element, be careful to
keep it clean and to avoid getting fingerprints on it.
38.2 1.0
34.0
ANGLE
ADJUSTMENT
RANGE
22.0
37.0 0.3
(8)
2
22.0
(REFERENCE PLANE OF
THE OPTICAL ELEMENT) 17
20.0 0.1
2-M3 DEPTH 3
(FOR HOLDING THE BLOCK IN PLACE)
TACCA0069EA
GParts Set A10455-02
This maintenance kit includes a set of screws and O-rings needed to assemble optical blocks.
Parts
Qty.
Optical blocks using parts at left
1
Hex wrench
A10030*, A10035-90, A10760, A10858, A10859, A11027, A11213, A11214, A11971
(5 pcs/set)
Thumb screws
4
1
A10030-01, A10030-02, A10032, A10033, A10034, A10035, A10036, A10038-02, A10039, A11026, A11971
Filter remove tool
A10033-90
Screw-ring tool
1
A11027
O-rings ( 15 mm)
2
A10030-01, A10032, A10033, A10034, A10035, A10036, A10038-02, A10039, A11026, A11971
O-rings ( 26 mm)
1
A10039
O-rings ( 28 mm)
1
A11026
Screws M2 × 12
Screws M2 × 16
10
10
10
10
10
10
10
5
A10030-01, A10030-02
A10039
Hex socket setscrews M2 × 2
Hex socket setscrews M2 × 4
Hex socket setscrews M3 × 4
Hex socket bolts M2 × 8
Hex socket bolts M2.6 × 6
Hex socket setscrews M3 × 16
Washers
A10035-90
A10030*
A11213
A10030*, A10035-90, A11026
A11214
A11027
3
A11026
* The A10030 is not currently sold.
19
CONNECTION EXAMPLES
GConnection Example Using Bifurcated Optical Fiber
Light from a light source is injected into one arm of the bifurcated optical fiber to irradiate a sample or measurement surface.
Fluorescence emitted from the sample or light reflected from the measurement surface is collected by the same optical fiber and then
detected by the PMT module through the other arm of the bifurcated optical fiber.
FILTER BLOCK A10033-xx
ADAPTER BLOCK A10030-01
PMT MODULE
FIBER ADAPTER BLOCK A10037
BANDPASS
LED
FILTER
TPMOC0213EA
GConnection Example Using the Beam Expander Block
The A10031 beam expander block is designed to narrow a 20-mm beam of collimated light to a diameter of 8 mm, the size of the
PMT module's photocathode; however, the expander block's use is not just limited to collimated light, it can also be used to converge
divergent light. For example, if you use the beam expander block to receive the divergent light from an optical fiber, the signal level
will be higher than if you had just used a PMT module.
BEAM EXPANDER BLOCK A10031
ADAPTER BLOCK A10030-01
OPTICAL FIBER
PMT MODULE
DISTANCE L1
DISTANCE L2
<Measurement Conditions>
Light source: Green LED
Optical fiber: NA=0.5. Core diameter=0.98 mm
Detection efficiency (Distance: 50 mm. Detection efficiency measured without beam expander block is normalized to 100.)
DISTANCE (L1, L2)
When Beam Expander is Connected (L1)
When Beam Expander is not Connected (L2)
50 mm
80 %
100 %
90 mm
59 %
59 %
250 mm 350 mm
28 %
13 %
20 %
7 %
TPMOC0214EA
GConnecting to a Microscope
The microscope port is designed so that the image is focused at 17.526 mm from the C-mount attachment location. When you
connect a C-mount adapter to a commercially sold 10-mm C-mount ring (male to female), the approximate location of the image
point is at the PMT module's photocathode (as shown in the figure on the left).
If the light converges to a diameter of 1 mm or less at the image point, do not match the location of the image point to the location of
the PMT module's photocathode; instead, expose the photocathode to a defocused light beam with a diameter of 1 mm or more.
MICRO-
SCOPE
MICRO-
SCOPE
C-MOUNT INTERCHANGEABLE
FILTER BLOCK
A11213-01 (SPACER)
C-MOUNT RING (MALE-FEMALE)
C-MOUNT RING
A9865
C-MOUNT RING
A9865
17.526
17.526
PMT
PMT
MODULE
MODULE
L*
7
10
L*
7
25
* L is the distance from the attached end of the PMT module to the photocathode.
TPMOC0216EA
20
GFluorescence Detection (Confocal)
For detecting fluorescence, it is convenient to use a laser with an optical fiber output. If you intend to focus the laser up to the
diffraction limit, use single-mode optical fiber. The infinity-corrected objective lens inside the beam aligner block collimates the light
beam emitted from the optical fiber. In this situation, you can collect the light efficiently if you use an objective lens with an NA that is
larger than that of the optical fiber. You can calculate the beam diameter from the optical fiber NA or the objective lens NA, whichever
is smaller, and the focal length of the objective lens.
The laser is reflected by the dichroic mirror, passes through the objective lens in the stage block, and excites the sample. The excited
fluorescence passes through the objective lens, dichroic mirror, and tube lens block and is focused on the position of the pinhole that
is installed in the pinhole block. This creates the confocal optical system. The light that passes through the pinhole is collimated by
the installed lens and is injected into the PMT module.
OPTICAL FIBER (FC CONNECTOR)
BEAM ALIGNER BLOCK A10760
LASER
OBJECTIVE LENS
(COMMERCIALLY AVAILABLE)
OBJECTIVE LENS
(COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213 (FOR THE CONNECTOR)
PINHOLE HOLDER BLOCK A11027
STAGE BLOCK
A10858
PMT MODULE
ADAPTER BLOCK A10030-01
FILTER BLOCK A10033-xx
PINHOLE (COMMERCIALLY AVAILABLE)
TUBE LENS BLOCK A10859-01
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
(FOR THE CONNECTOR)
C-MOUNT INTERCHANGEABLE
DICHROIC BLOCK A11214
C-MOUNT DOUBLE MALE THREAD RING
(SUPPLIED WITH A10859-01)
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
TPMOC0217EA
Technical Guide
The performance of the confocal optical system is determined by the size of the pinhole. The Airy disk (diameter) is used as the
reference. The following equation is used to calculate the Airy disk. The system magnification is determined by the focal length of the
observation-side objective lens and the focal length of the tube lens block.
λ
Airy disk (diameter) = 1.22 × M ×
NA
Focal length of the tube lens block
M: System magnification =
Focal length of the observation-side objective lens*
λ
: Fluorescence wavelength
NA: NA of the observation-side objective lens
* If the objective lens focal distance on the observation side is unknown, please contact the lens manufacturer.
If spatial resolution is important, use a pinhole whose size is close to the size of the Airy disk. If you are only measuring fluorescence
intensity, make the pinhole larger than the Airy disk; this will lower the spatial resolution, but it will also increase the signal level.
GConnection Example Using an Objective Lens and the Tube Lens Block
An infinity-corrected objective lens is connected to the A10859 tube lens block with a microscope objective lens mount, which has
threads for a M20.32 × 0.706 mm objective lens and a C-mount male thread. Next, the tube lens block is connected to an imaging
camera, such as an ICCD camera. You can adjust the focus using the manual focusing ring on the tube lens block.
TUBE LENS BLOCK A10859
OBJECTIVE
LENS
ICCD
CAMERA
MICROSCOPE OBJECTIVE LENS MOUNT *
* Example: Edmund Optics
(COMMERCIALLY AVAILABLE)
Microscope objective lens mount
TPMOC0215EA
21
CONNECTION EXAMPLES
G Imaging System Connection Example
Coaxial Incident Illumination Method
Illuminate the sample with a fiber bundle connected to a xenon arc lamp. You can attach the fiber bundle easily by using a
commercially sold C-mount light guide mount. To excite the sample, put an excitation filter that matches its fluorescent dye into the
A11213-01 C-mount interchangeable filter block. If the light from the fiber bundle is not diffused enough, you can illuminate the
sample evenly by inserting a holographic or other type of diffuser.
The dichroic mirror reflects the excitation light. Then, the objective lens of the A10858 stage block focuses the light onto the sample,
which is excited by the light. The fluorescence emitted from the sample passes through the dichroic mirror and the fluorescence filter
and is focused onto the imaging camera by the lens inside the A10859-01 tube lens block.
LIGHT GUIDE A10014-35-0110
XENON LAMP
LIGHT GUIDE ADAPTER*
(COMMERCIALLY AVAILABLE)
L9588-03
EXCITATION FILTER
(COMMERCIALLY AVAILABLE)
C-MOUNT LIGHT GUIDE MOUNT*
(COMMERCIALLY AVAILABLE)
HOLOGRAPHIC DIFFUSER*
(COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK
A11213-01
FLUORESCENCE FILTER (COMMERCIALLY AVAILABLE)
EM-CCD
or
ICCD CAMERA
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
TUBE LENS BLOCK A10859-01
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR)
STAGE BLOCK A10858
* Example: Edmund Optics
•C-Thread Light Guide Mounting Adapter
•Fiber Optic Light Guide Adapter SX-6
•Holographic Diffuser: 25 mm Type
TPMOC0218EA
Transmitted Illumination Method
This is an imaging example that uses transmitted illumination. The image of the sample is focused onto an imaging camera, such as
a CCD camera, by an infinity-corrected objective lens and the A10859-01 tube lens block. A convenient way to illuminate the sample
is to use a commercially sold LED illuminator.
C-MOUNT DOUBLE MALE THREAD RING
(SUPPLIED WITH A10859-01)
CCD
CAMERA
OBJECTIVE LENS
(COMMERCIALLY
TUBE LENS BLOCK
AVAILABLE)
A10859-01
SAMPLE
LED LIGHT
SOURCE
STAGE BLOCK A10858
TPMOC0219JA
22
GEmission Detection
Collimated light such as chemiluminescence and bioluminescence captured by an infinity-corrected lens is reduced to 1/2.5 by using
the A10031 beam expander and is guided to the PMT module with an effective diameter of 8 mm. A filter block is added when
detesting light at a specific wavelength.
C-MOUNT DOUBLE MALE THREAD RING
(SUPPLIED WITH A10031)
SAMPLE
BEAM EXPANDER BLOCK A10031
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
PMT MODULE
ADAPTER BLOCK A10030-01
STAGE BLOCK A10858
FILTER BLOCK A10033-xx
TPMOC0255EA
GExample of Connection to a Mini-Spectrometer
The laser beam emitted from the optical fiber is collimated by the infinity-corrected objective lens inside the A10760 beam aligner
block. The dichroic mirror inside the A11214 C-mount interchangeable dichroic mirror block reflects the collimated laser, which then
passes through the objective lens in the A10858 stage block and illuminates the sample. The fluorescence from the excited sample is
converged by the objective lens and then passes through the dichroic mirror inside the A11214. The fluorescence is then converged
further by the A10031 beam expander block. The lens inside the fiber adapter block converges the light into the optical fiber and
guides it to the mini-spectrometer, where it is measured. If you want to remove the excitation light, place the A10033 filter block
between the beam expander block and the A10037-01 fiber adapter block. The fiber adapter block does not have focusing
capabilities, so we recommend that you connect the fiber adapter block and the mini-spectrometer with an optical fiber whose core
diameter is at least 600 µm.
OPTICAL FIBER (FC CONNECTOR)
BEAM ALIGNER BLOCK A10760
LASER
OBJECTIVE LENS (COMMERCIALLY AVAILABLE)
C-MOUNT DOUBLE MALE THREAD RING
(SUPPLIED WITH A10031)
C-MOUNT INTERCHANGEABLE FILTER BLOCK
A11213 (FOR THE CONNECTOR)
JOINT BLOCK
A10038-02
STAGE BLOCK
A10858
FIBER ADAPTER BLOCK
A10037-01
OPTICAL FIBER (SMA CONNECTOR)
(RECOMMENDED FIBER CORE
DIAMETER : 600 µm or more)
MINI
SPECTORO-
METER*
BEAM EXPANDER BLOCK A10031
C-MOUNT INTERCHANGEABLE
DICHROIC BLOCK A11214
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
* There is a separate catalog for mini-spectrometers.
Please refer to that catalog for more information.
TPMOC0221EA
23
CONNECTION EXAMPLES
GSimultaneous Measurement with a PMT Module and an Imaging Camera
The following is an example of simultaneous measurement in which the two A10921 objective lens mounts are attached to the
A10858 stage block. The lower (inverted) part excites the sample using laser light and measures the fluorescence of the sample
using the PMT module. The upper (upright) part illuminates the observation area using a xenon arc lamp and uses an imaging
camera to observe the observation area and the fluorescence signal.
A plano-convex lens and a fluorescence filter are installed in front of the PMT module to isolate the fluorescence. The plano-convex
lens converges the beam of light. If this causes a problem in the fluorescence filter's wavelength shift, place the filter in front of the
plano-convex lens.
So that there is no interference with the PMT module's measurement, use a wavelength for the imaging camera's illumination that is
different from both the excitation wavelength and the fluorescence wavelength. For example, if you use a 488 nm laser, use a DM505
dichroic mirror for the PMT module and a 530/40 bandpass filter for the emission filter. On the imaging camera side, use a 600 nm
longpass filter for illumination and put a laser-blocking filter in front of the camera.
LIGHT GUIDE A10014-35-0110
XENON LAMP
LIGHT GUIDE ADAPTER*
L9588-03
(COMMERCIALLY AVAILABLE)
C-MOUNT LIGHT GUIDE MOUNT*
(COMMERCIALLY AVAILABLE)
600 nm LONGPASS FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213-01
TUBE LENS BLOCK A10859-01
STAGE BLOCK A10858
OBJECTIVE
LENS MOUNT
A10921
EM-CCD
or
ICCD CAMERA
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
LASER-BLOCKING FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213
C-MOUNT CUBE BEAMSPLITTER* (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213 (FOR THE CONNECTOR)
C-MOUNT INTERCHANGEABLE DICHROIC BLOCK A11214
DICHROIC MIRROR (COMMERCIALLY AVAILABLE)
C-MOUNT RING A9865
OBJECTIVE LENS
(COMMERCIALLY
AVAILABLE)
OBJECTIVE
LENS MOUNT
A10921
PMT MODULE
FLUORESCENCE FILTER (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK
A11213-01
C-MOUNT INTERCHANGEABLE
FILTER BLOCK A11213
(FOR THE CONNECTOR)
PLANO-CONVEX LENS (COMMERCIALLY AVAILABLE)
C-MOUNT INTERCHANGEABLE FILTER BLOCK A11213
BEAM ALIGNER BLOCK A10760
OBJECTIVE LENS
(COMMERCIALLY AVAILABLE)
OPTICAL FIBER (FC CONNECTOR)
LASER
* Example: Edmund Optics
•C-Thread Light Guide Mounting Adapter
•Fiber Optic Light Guide Adapter
•C-Mounted Cube Beamsplitter
NOTE: The A10858 stage block comes with one A10921 objective lens mount.
To perform simultaneous measurement as shown above, you will need to purchase an additional A10921.
TPMOC0222EA
24
GFluorescence Dye and Connecting Examples
1
GTransmittance
TACCB0090EA
100
633 nm 532 nm
A10034-04
DM 660
90
80
70
60
50
40
30
20
10
0
A10033-05
575/30 nm
A10033-06
700/60 nm
OBJECTIVE
LENS
Cy3™
Cy5™
Alexa® 546
Alexa® 647
SAMPLE
400
500
600
700
800
TACCC0128EA
WAVELENGTH (nm)
2
GTransmittance
TACCB0091EA
100
90
80
70
60
50
40
30
20
10
0
A10034-01
505 nm
FLUORESCENCE
EXCITATION
LIGHT
A10033-04
460/60 nm
A10033-01
530/40 nm
405 nm
CASY
FITC
DAPI
Hoechst
400
500
600
700
800
TACCC0129EA
WAVELENGTH (nm)
3
GTransmittance
100 TACCB0092EA
90
80
70
60
50
40
30
20
10
0
A10033-62
500 nmLP
A10034-02
DM 565
A10034-03
DM 600
A10034-04
DM 660
FLUORESCENCE
EXCITATION
LIGHT
488 nm
A10033-01
530/40 nm
A10033-05
575/30 nm
A10033-03
630/60 nm
A10033-06
700/60 nm
®
PE
CASY
FITC
GFP
Texas Red
Cy5™
7AAD
PI
400
500
600
700
800
TACCC0130EA
WAVELENGTH (nm)
25
RELATED PRODUCTS
GStabilized Light Sources For PMT L11494 Series
The majority of these devices are LED light sources that have been
developed for visual-sensitive compensation of PMTs and PMT
modules.
Because the amount of light generated by the LED is monitored and
controlled by a photodiode, these devices always generate stable light
of approximately 1 pW.
SPECIFICATIONS
L11494-430 L11494-470 L11494-525 L11494-660 Unit
Parameter
Peak Wavelength of
Generated Light
FWHM
428
65
465
26
522
35
660
30
nm
nm
Wavelength Distribution of Generated Light (Typical)
GL11494-430
GL11494-470
Life Characteristic
TACCB0114EA
1
TACCB0115EA
1
TACCB0120EA
1.2
1
0.8
0.6
0.4
0.2
0
0.8
0.6
0.4
0.2
0
0.8
0.6
0.4
0.2
0
350 400 450 500 550 600 650 700 750
350 400 450 500 550 600 650 700 750
0
6
12
18
24
30
WAVELENGTH (nm)
WAVELENGTH (nm)
TIME (h)
GL11494-525
GL11494-660
Temperature Characteristic
TACCB0116EA
1
TACCB0119EA
TACCB0121EA
2
1
0.8
0.6
0.4
0.2
0
1.5
1
0.8
0.6
0.4
0.2
0
0.5
0
-0.5
-1
-1.5
-2
350 400 450 500 550 600 650 700 750
350 400 450 500 550 600 650 700 750
0
5
10
15
20
25
30
35
40
45
50
WAVELENGTH (nm)
WAVELENGTH (nm)
TEMPERATURE (°C)
Connection Example
Dimensional Outlines (Unit: mm)
24
STABILIZED LIGHT SOURCE
L11494-xxx
8.5
12
2-M2
JOINT BLOCK A10038-01
PMT MODULE
EMISSION AREA
7
ON
OFF
ON
(Low intensity)
(High intensity)
14
BATTERY SR41
SWITCH
ADAPTER BLOCK
A10030-01
* There is a separate catalog for stabilized light sources.
Please refer to that catalog for more information.
TACCA0311EA
26
RELATED PRODUCTS
GPhotosensor Modules H10799 Series
The H10799 series modules are cable output modules with S1337-
1010 series photodiodes and current-to-voltage conversion amplifiers.
You can connect it easily to optical blocks and use it as a photodetector.
SPECIFICATIONS
H10799 Series
-101 -002
Parameter
Suffix
-001
-102
Spectral Response
Input Voltage
190 nm / 320 nm to 1100 nm (See spectral response)
+5 V
Max. Input Voltage
Max. Output Signal Voltage
Current-to-voltage
Conversion Factor /
Frequency Bandwidth
Effective Area
+5.5 V
+4.5 V
0.1 V/nA / DC to 100 Hz
1 V/nA / DC to 10 Hz
0.01 V/nA / DC to 1 kHz
(Switchable)
* Using example: P.6
8 mm
Spectral Response
Operating Ambient Temperature
Storage Temperature*
Internal photodiodes
* No condensation
*
+5 °C to +50 °C
-20 °C to +50 °C
S1337-1010BQ S1337-1010BR S1337-1010BQ S1337-1010BR
TPMOB0213EA
1
H10799-101
H10799-001
Dimensional Outlines (Unit: mm)
0.1
H10799-102
14.0 0.2
EFFECTIVE AREA
8
H10799-002
0.01
MOUNTING HOLES FOR OPTIONS 4-M2
GND
11
H10799-002 and H10799-102 are the values when
the current-to-voltage conversion factor is 0.1 V/nA
and the frequency bandwidth is DC to 100 Hz.
: AWG26 (BLACK)
Vcc LOW VOLTAGE INPUT: AWG26 (RED, +5 V)
SIGNAL OUTPUT : RG-174/U
0.001
200
300
400
500
600
700
800
900
1000 1100
50.0 0.5
450 20
WAVELENGTH (nm)
TPMOA0048EA
GICCD Camera Units C10054 Series
C10054 series cameras boast high speed and high sensitivity thanks to
their built-in image intensifiers and high-speed electronic shutters
(min. 5 ns).
SPECIFICATIONS
Spectral Response Minimum Photocathode
Limiting Resolution
(TV Lines)
Type No.
(mm)
Illuminance (lx)
1 × 10-6
4 × 10-7
4 × 10-5
4 × 10-7
1 × 10-6
4 × 10-7
C10054-x1
C10054-x2
C10054-x3
C10054-x4
C10054-x5
C10054-x6
470
450
480
420
470
450
280 to 720
185 to 900
370 to 920
* Image intensifier: an imaging device with high light-intensification capabilities and
high-speed gate operation (shutter operation).
* Using example: P.21, P22, P24
* There are separate catalogs for image intensifiers and ICCD cameras. Please
refer to those catalogs for more information.
27
Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult with our sales office.
Information furnished by HAMAMATSU is believed to be reliable. However, no responsibility is assumed for possible inaccuracies or omissions. Specifications are
subject to change without notice. No patent rights are granted to any of the circuits described herein. ©2014 Hamamatsu Photonics K.K.
www.hamamatsu.com
HAMAMATSU PHOTONICS K.K.
HAMAMATSU PHOTONICS K.K., Electron Tube Division
314-5, Shimokanzo, Iwata City, Shizuoka Pref., 438-0193, Japan, Telephone: (81)539/62-5248, Fax: (81)539/62-2205
U.S.A.: Hamamatsu Corporation: 360 Foothill Road, Bridgewater. N.J. 08807-0910, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: usa@hamamatsu.com
Germany: Hamamatsu Photonics Deutschland GmbH: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-2658 E-mail: info@hamamatsu.de
France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: infos@hamamatsu.fr
United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court, 10 Tewin Road, Welwyn Garden City, Hertfordshire AL7 1BW, United Kingdom, Telephone: (44)1707-294888, Fax: (44)1707-325777 E-mail: info@hamamatsu.co.uk
North Europe: Hamamatsu Photonics Norden AB: Torshamnsgatan 35 SE-164 40 Kista, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: info@hamamatsu.se
TPMO1058E01
FEB. 2014 IP
Italy: Hamamatsu Photonics Italia S.r.l.: Strada della Moia, 1 int. 6, 20020 Arese (Milano), Italy, Telephone: (39)02-93581733, Fax: (39)02-93581741 E-mail: info@hamamatsu.it
China: Hamamatsu Photonics (China) Co., Ltd.: B1201 Jiaming Center, No.27 Dongsanhuan Beilu, Chaoyang District, Beijing 100020, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: hpc@hamamatsu.com.cn
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